Based on differentially regulated pathways, we developed a reprogramming protocol to induce high stemness in presenescent ANPGs (i.e., from C57Bl6 mouse). A transcriptomic comparison of traditionally low-stemness ANPGs, isolated from C57Bl/6 and A/J mice at early passages, and high-stemness phoenix ANPGs was performed, allowing the identification of several differentially expressed pathways. ![]() In the present study, we aim at identifying the signaling pathways responsible for the intrinsic high stemness of phoenix ANPGs. We previously identified and characterized the phoenix auditory neuroprogenitors (ANPGs) as highly proliferative progenitor cells isolated from the A/J mouse cochlea. Currently, the research field mainly relies on primary organotypic inner ear cultures, resulting in high variability, low throughput, high associated costs and ethical concerns. As of today, a main limitation for the development of otoprotective or otoregenerative therapies is the lack of efficient preclinical models compatible with high-throughput screening of drug candidates. In contrast to birds, fish and amphibians, the mammalian inner ear is virtually unable to regenerate due to the limited stemness of auditory progenitors, and no causal treatment is able to prevent or reverse hearing loss. Both genetic and environmental factors (i.e., noise overexposure, ototoxic drug treatment and ageing), promote the irreversible degeneration of cochlear hair cells and associated auditory neurons, leading to sensorineural hearing loss. Hearing loss affects over 460 million people worldwide and is a major socioeconomic burden. Authors may use MDPI'sĮnglish editing service prior to publication or during author revisions. ![]() Submitted papers should be well formatted and use good English. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Please visit the Instructions for Authors page before submitting a manuscript. Cells is an international peer-reviewed open access semimonthly journal published by MDPI. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. All manuscripts are thoroughly refereed through a single-blind peer-review process. Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website. Research articles, review articles as well as short communications are invited. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. All submissions that pass pre-check are peer-reviewed. Manuscripts can be submitted until the deadline. Once you are registered, click here to go to the submission form. Manuscripts should be submitted online at by registering and logging in to this website. We look forward to publishing your exciting and thought-provoking research communications. iPSC-based transdifferentiation toward cochlear hair cells. iPSC-based disease modeling to study the effect(s) of environmental/toxic compound/idiopathic factors ħ. Emerging therapeutic application of CRISPR and iPSC for hereditary deafness Ħ. iPSC differentiation protocols toward sensory neurons and other inner ear components ĥ. 3D-organoid-based disease modeling of hereditary deafness Ĥ. Cellular and molecular biology of inner ear development ģ. iPSC-based investigation of inner ear development Ģ.I am delighted to invite you to publish your research articles and review papers in this issue focused on the following domains. ![]() This Special Issue is designed to stimulate an understanding of sensorineural hearing loss and provide a platform to exchange new concepts and ideas, focusing on induced pluripotent stem cells (iPSCs) and 3D-organoid-based disease modeling, genetic and epigenetic perturbation through CRISPR, and emerging innovative techniques and protocols. It is of utmost importance to find an effective therapeutic intervention with the available cutting-edge research-based tools that can cure or halt disease progression. Hearing loss is the most common sensorineural disorder affecting around 6.1% of the world population.
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